Resumos Aprovados 2025

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HA011 - Hatton
Área: 5 - Materiais Dentários

Apresentação: 04/09 - Horário: 08h30 às 17h30 - Local: Salão Cinza

Nanostructured silver vanadate decorated with silver nanoparticles: multimodal approach against SARS-CoV-2 and oral biofilms
João Marcos Carvalho Silva, Izabela Ferreira, Daniel Watterson, Naphak Modhiran, Alberto Anastácio Amarilla, Marco Antonio Schiavon, Oswaldo Luiz Alves, Andréa Cândido Dos Reis
Materiais Dentários e Prótese UNIVERSIDADE DE SÃO PAULO - RIBEIRÃO PRETO
Conflito de interesse: Não há conflito de interesse

This study aimed to synthesize nanostructured silver vanadate decorated with silver nanoparticles (β-AgVO₃), evaluate its antimicrobial and antiviral activities, and develop a hydrogel to assess its antibiofilm potential. β-AgVO₃ was synthesized via chemical reduction and characterized by transmission electron microscopy (TEM) and X-ray diffraction (XRD). Antiviral activity against SARS-CoV-2 (Wuhan) was evaluated by plaque reduction neutralization in Vero cells, with REGN10933 and 6A11 monoclonal antibodies as controls (n = 9). Antimicrobial activity against planktonic Candida albicans, Streptococcus mutans, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Enterococcus faecalis was assessed by minimum inhibitory concentration (MIC, n = 3). Hydrogels were formulated with no antimicrobial (G0), 0.12% w/v β-AgVO₃ (G1), and 0.12% w/v chlorhexidine (G2). Antibiofilm activity was tested in 2 models: a 5-species (P. aeruginosa, E. coli, E. faecalis, S. aureus, C. albicans) and a 3-species (C. albicans, S. mutans, and S. aureus) by colony forming unit (CFU) (n = 8). Were applied the 1-way ANOVA followed by Tukey's post hoc. TEM revealed β-AgVO₃ as nanowires decorated with nanoparticles and XRD identified its crystalline structure. REGN10933 inhibited viral entry (0.65 µg/mL), and β-AgVO₃ achieved 75% inhibition (2 mg/mL, p = 0.001). MIC values were 125-250 µg/mL. In the 5-species model, G1 eradicated P. aeruginosa, E. coli, S. aureus, and C. albicans. In the 3-species model, G1 was effective against S. mutans and S. aureus (p < 0.05).

It is concluded that β-AgVO₃ has activity against SARS-CoV-2 and planktonic microorganisms and exhibits antibiofilm effect when incorporated into hydrogel.

(Apoio: CNPq - FAPESP N° 314132/2021-0 | FAPs - FAPESP N° 23/11348-0)

HA012 - Hatton
Área: 5 - Materiais Dentários

Apresentação: 04/09 - Horário: 08h30 às 17h30 - Local: Salão Cinza

HEALING SMARTER: ENHANCEMENTS IN STABILITY FOR THE CLINICAL TRANSLATION OF SELF-HEALING COMPOSITES
Maria Eduarda Lima do Nascimento Marinho, Sivashankari Rajasekaran, Bao Huynh, Carmem Pfeifer, Ana Paula Piovezan Fugolin, Giana da Silveira Lima
UNIVERSIDADE FEDERAL DE PELOTAS
Conflito de interesse: Não há conflito de interesse

The clinical translation of self-healing composites based on microcapsule technology remains limited due to concerns about material stability, potential toxicity from degradation products and inconsistent healing performance. This study aimed to identify factors that hinder translation by evaluating the structural and thermal stability of poly(urea-formaldehyde) (PUF) microcapsules and assessing how healing agent, shell structure, capsule size, storage conditions and benzoyl peroxide (BPO) concentration affect healing efficiency. Healing agents (100% TEGDMA or 50% BisGMA : 50% TEGDMA) were encapsulated in PUF or melamine-modified PUF (PUMF) capsules. Characterization included optical microscopy, differential scanning calorimetry and formaldehyde quantification. Capsules were aged at 4°C and 37°C for 6 months. PUMF 50B:50T capsules were selected (100, 200, 300 µm) for further testing in an experimental dental resin containing bisacylphosphine oxide (BAPO) and variable BPO concentrations (0.5, 2, 5%). Data were analyzed using one-way ANOVA and Tukey's test (α=0.05). Healing efficiency (>20%) shows consistently higher values across all sizes at 5% BPO. PUMF capsules showed significantly higher thermal stability, structural integrity, and lower core leakage. Melamine incorporation promoted surface roughness and increased microcapsules stability regardless of the storage conditions. Capsules containing melamine released more formaldehyde in the first week, but levels dropped significantly afterward in all groups.

Overall, PUMF 50B:50T microcapsules demonstrated superior stability and consistent healing performance, maintaining functionality and supporting their translation from bench to bedside.

(Apoio: CNPq N° 407841/2023-7; 141072/2022-9 | FAPERGS N° 21/2551-0002074-1 | NIH/NIDCR N° R00 DE028876)

HA013 - Hatton
Área: 5 - Materiais Dentários

Apresentação: 04/09 - Horário: 08h30 às 17h30 - Local: Salão Cinza

3D printing of Ti6Al4V alloy at different angles: Topographical and crystalline characteristics
Lívia Maiumi Uehara, Rodolfo Lisboa Batalha, Lucas Barcelos Otani, Andréa Cândido Dos Reis
Materiais Dentários e Prótese UNIVERSIDADE DE SÃO PAULO - RIBEIRÃO PRETO
Conflito de interesse: Não há conflito de interesse

The aim of this study was to evaluate the microstructural characteristics of the topography and crystallinity of Ti6Al4V surfaces obtained by Powder Bed Fusion - Laser Beam additive manufacturing technique (PBF-LB AM) at two printing angles. The samples were grouped into: Wrought discs (WD); Additive-manufactured Discs 0º (AD0º); Additive-manufactured Discs 45º (AD45º). Polishing was performed to enable analyses incompatible with rough surfaces. Surface characterization (n =1) was conducted using atomic force microscopy (AFM); scanning electron microscopy (SEM); X-ray diffraction (XRD); and electron backscatter diffraction (EDBS). AFM showed greater height difference for WD (469.1nm), with amplitude between 1.9nm and 2nm. The lowest surface potential values were found in AD45º (387.3mV to 273.7mV SEM micrographs revealed a flat surface with grooves in WD, and spherical particles suggestive of unmelted metal powder in AD0º and AD45º. XRD (2θ/θ) identified the α-phase of titanium (Ti) in all samples. EBSD mapping of WD showed rounded grain morphology and heterogeneous density, associated with greater crystallographic texture, while AD0º and AD45º had elongated, needle-shaped grains and more homogeneous density, indicating lower crystallographic texture.

It is concluded that PBF-LB AM produces Ti6Al4V surfaces with different microstructural characteristics from the conventional method and which have the biomedical potential. Further transdisciplinary studies are encouraged to explore the influence of printed titanium microstructure on bone cells and microorganisms, contributing to future clinical application.

(Apoio: Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) N° 2023/11966-5 )

HA014 - Hatton
Área: 7 - Patologia Oral

Apresentação: 04/09 - Horário: 08h30 às 17h30 - Local: Salão Cinza

EFFECTS OF SAHA AND EMETINE COMBINED WITH CISPLATIN CHEMOTHERAPY IN ORAL CANCER: A PATIENT-DERIVED XENOGRAFT MOUSE MODEL STUDY
Sebastião Silvério de Sousa Neto, Brendo Vinicius Rodrigues Louredo, Luan César da Silva, Mateus José Dutra, Vivian Petersen Wagner, Manoela Domingues Martins, Rogério Moraes de Castilho, Pablo Agustin Vargas
Diagnóstico Oral FACULDADE DE ODONTOLOGIA DE PIRACICABA
Conflito de interesse: Não há conflito de interesse

The aim of this study was to evaluate the effects of histone deacetylase inhibitors (HDACi - SAHA) and IκBα phosphorylation inhibitors (emetine) combined with cisplatin chemotherapy in the treatment of oral cancer. Patient-derived xenografts (PDXs) were obtained from six patients. Fresh tumor fragments (5×5 mm) were subcutaneously implanted into 28 NOD/SCID mice (PDX0), expanded to PDX2, and 39 tumor-bearing animals (200-400 mm²) were randomized into eight groups: controls (saline or DMSO), cisplatin alone, SAHA and/or emetine combinations, and monotherapies. This study was approved by the Committee on the Use and Care of Animals. After four weeks of intraperitoneal treatment, animals were euthanized, and tumors and organs were collected for morphological and cell cycle analyses. Engraftment success ranged from 57% in PDX0 to 80% in PDX2, with a latency period of 60-120 days. Tumor growth control was greater in the cisplatin + emetine and/or SAHA groups. Cell cycle analysis showed increased S-G2-M phases in sensitizer-treated animals. Although histological changes were observed in the spleen, heart, lungs, liver, and kidneys, no statistically significant differences remained after correction for multiple comparisons. PDX0 tumors showed higher SMA expression in stromal fibroblasts and an increased Ki-67 index, while CD4, CD8, CD31, and CD34 showed lower expression compared to donor tumors.

These findings suggest that SAHA and emetine may enhance cisplatin efficacy without increasing systemic toxicity, supporting further studies with reduced cisplatin dosage. Furthermore, our data support the use of PDX models as a robust preclinical platform for identifying therapeutic targets and developing novel anticancer drugs.

(Apoio: FAPs - FAPESP N° 2023/13797-6)

HA015 - Hatton
Área: 7 - Patologia Oral

Apresentação: 04/09 - Horário: 08h30 às 17h30 - Local: Salão Cinza

Diagnostic significance of Siglec-5 in Sjögren disease
Fernanda Luiza Araújo de Lima Castro, Laiz Fernandes Mendes Nunes, Fernanda Aragão Felix, Sicília Rezende Oliveira, Mariana Silveira Souza, Maurício Augusto Aquino de Castro, Sílvia Ferreira de Sousa, Tarcília Aparecida da Silva
Clínica, Patologia e Cirurgia UNIVERSIDADE FEDERAL DE MINAS GERAIS
Conflito de interesse: Não há conflito de interesse

Sjögren disease (SD) is a disorder that leads to multiple organic dysfunctions, and its etiology remains not completely understood. In this context, the study of novel biomarkers provides valuable insights into its mechanisms, diagnosis, and treatment. This study aimed to investigate the diagnostic accuracy of the sialic acid-binding immunoglobulin-like lectin 5 (siglec-5) expression in minor salivary gland (MSG) and saliva to identify SD. Individuals presenting complaints of dry mouth and/or dry eyes who met the diagnostic criteria for SD (n=109) were included. Patients with these symptoms who did not meet the diagnostic criteria (n=41) were included as controls (non-Sjögren sicca, nSS). The concentration of siglec-5 and a panel of salivary inflammatory markers (IL-6, IL-8, INF-γ, IgA, IgG, nitric oxide and neutrophil extracellular traps - NETs) was assessed. Immunohistochemical analysis of siglec-5, CD20 and CD3 was done in MSG. Significantly higher salivary concentrations of siglec-5, IgA, IgG, INF-γ, nitric oxide and NETs were observed in the SD. Immunostaining for siglec-5 was also more pronounced in SD, with siglec-5 being predominantly expressed by mononuclear inflammatory cells. SD group exhibited more pronounced CD20 and CD3 positivity than nSS. Salivary siglec-5 positively correlated with IL-8, IL-6, IgA, IgG, and NETs. ROC curve showed that tissue infiltration of siglec-5 has superior diagnostic accuracy than salivary siglec-5 and ocular tests for SD diagnostic.

Findings indicate that siglec-5 positivity may be associated with overall inflammation suggesting its participation in the SD mechanisms and potential marker for diagnosis.

(Apoio: FAPEMIG N° APQ-01318-21 e APQ-03440-22 | CAPES - FAPEMIG | CNPq)

HA016 - Hatton
Área: 8 - Periodontia

Apresentação: 04/09 - Horário: 08h30 às 17h30 - Local: Salão Cinza

Effect of Bioactive Gel with S-PRG on Gingival Health and Salivary Microbiota in Children: Preliminary Study
Tuany Rayra Pinto Lisboa Dias, Alana Gabrieli Vouk, Eduarda Fagherazzi, Juliana Feltrin de Souza, Yasmine Mendes Pupo, Geisla Mary Silva Soares
UNIVERSIDADE FEDERAL DO PARANÁ
Conflito de interesse: Não há conflito de interesse

The objective of this randomized clinical study was to evaluate the clinical and microbiological effects of a bioactive gel containing surface pre-reacted glass-ionomer (S-PRG) particles. Twenty-one patients treated at the Pediatric Dentistry Clinic of the Federal University of Paraná and the Ecumenical Foundation for Exceptional Protection in Curitiba/PR were included. Saliva samples were collected, and gingival health was assessed using the Modified Gingival Index (MGI). Subsequently, patients received prophylaxis with pumice and 2% fluoride gel (control group) (n=10) or S-PRG gel (Pro-care gel S-PRG - Shofu Inc., Japan) (n=11). Prophylaxis and gel applications were repeated weekly for 4 weeks, and assessments were repeated after 3 months. Saliva samples were inoculated on Brain Heart Infusion Agar plates + 5% sheep blood. Plates were incubated at 37ºC under anaerobic conditions for 7 days and then evaluated for the number of colony-forming units (CFUs). Statistical differences were assessed using the Mann-Whitney U test. The mean age of the children was 9 and 11 years in the control and test groups, respectively. The mean ± SD MGI observed in the S-PRG gel group decreased from 1.11 ± 1.04 at baseline to 0.64 ± 0.33 (p<0.05), while in the control group, it decreased from 0.87 ± 0.53 to 0.51 ± 0.32 (p<0.05). No significant differences in MGI were observed between the groups (p>0.05). The mean ± SD CFUs in the control and test groups were 1,050 ± 1,821 and 1,357 ± 1,428 at baseline, and 981 ± 1,066 and 573 ± 651 after 3 months, respectively (p>0.05).

Therefore, the use of S-PRG gel reduced gingival inflammation and colony counts similarly to the use of pumice and fluoride gel.

HA017 - Hatton
Área: 10 - Implantodontia básica e biomateriais

Apresentação: 04/09 - Horário: 08h30 às 17h30 - Local: Salão Cinza

Effects of Vitex agnus-castus L. phytoestrogen administration on bone repair around loaded implants in ovariectomized rats
Maria Carolina Coelho, Antonio Nanci, Karina Fittipaldi Bombonato Prado
Biologia Básica e Oral UNIVERSIDADE DE SÃO PAULO - RIBEIRÃO PRETO
Conflito de interesse: Não há conflito de interesse

Osteoporosis impairs bone homeostasis by increasing bone resorption and reducing the deposition of mineralized matrix. Current treatments often cause adverse side effects, highlighting the need for effective alternatives. Phytoestrogens have attracted interest due to their estrogen-like effects, stimulating bone formation, reducing resorption, and counteracting oxidative stress by neutralizing reactive oxygen species. Osteoporotic fractures often require implants and bone substitutes, making osseointegration crucial. This study aimed to evaluate the effect of Vitex agnus-castus (VAC) on bone repair around titanium implants in an osteoporotic rat model. Ovariectomized animals were divided into control groups (SHAM and SHAM+VAC) and ovariectomized groups (OVX and OVX+VAC). VAC was administered orally three times per week (200 mg/kg) for 28 days. Implants were inserted into the tibia after 14 days, followed by controlled micromovements of 1.5 N once or twice daily for 7 days. Animals were euthanized on day 28, and the tibiae were analyzed by histomorphometry. Statistical analysis (p < 0.05) showed no significant differences in bone formation with once-daily loading. However, twice-daily loading altered the bone-implant distance and cartilage formation between implant threads in all groups. Bone formation in the tibia did not increase significantly in the VAC-treated groups, regardless of the presence of osteoporosis or the loading protocol.

These findings suggest that, at the tested concentration, VAC does not significantly influence bone metabolism in osteoporotic conditions associated with controlled loading around titanium implants.

(Apoio: FAPs - Fapesp N° 2023/06393-6)