HA006 - Hatton
Área: 4 - Odontopediatria

Apresentação: 10/09 (Sexta-feira) - Horário: 10h30 - 12h00 - Sala: 7

Selective Outcome Reporting (SOR) in Pediatric Dentistry restorative treatment Randomized Clinical Trials - A systematic Review
Elagami RA, Tedesco TK, Pannuti CM, Seabra G, Braga MM, Mendes FM, Raggio DP
Odontopediatria e Ortodontia - UNIVERSIDADE DE SÃO PAULO - SÃO PAULO
Conflito de interesse: Não há conflito de interesse

We aimed to evaluate the prevalence of SOR in the randomized clinical trials focusing on restorative treatment in primary molars. We searched for RCTs protocol registries in the ClinicalTrials.gov and WHO platform up to April/2021. We included any study classified as an RCT, with two or more experimental arms that evaluated any restorative treatment on primary teeth. The registries that did not result in any publications were excluded. Our search strategy identified 294 registries and 31 publications were included for data extraction. We have also calculated the median of journals' impact factors. Chi-square test was performed with 5% significance level. Our results found that the prevalence of SOR was identified in 19 papers (61.3%). The most common reason was a discrepancy in the primary outcome timeframe (48.4%). Twenty-six (83.9%) of the trials were registered retrospectively. Also, a discrepancy in sponsorship was identified in 22 (71%) of the trials. SOR was associated with the discrepancy in the follow-up period (p=0.003), with no association with registration timing (p=0.948). The impact factor of the journals ranged from 0.213 - 5.79. We found SOR in 9 (29%) of the six high impact factor journals and 10 (32.3%) of the eight with low impact.
Consequently, we emphasized the importance of transparency in RCT focusing on restorative care in children, including properly prospective registration and declaration of any discrepancy in the final manuscript, helping to avoid spin in science.
(Apoio: CAPES  N° 001)

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HA007 - Hatton
Área: 4 - Odontopediatria

Apresentação: 10/09 (Sexta-feira) - Horário: 10h30 - 12h00 - Sala: 7

Probiotic: in vivo evaluation of the effect on progression of periapical lesions, through microscopic analysis
Araujo LDC, Silva RAB, Messora MR, Furlaneto FAC, Salvador SLS, Nelson-Filho P, Pucinelli CM, Silva LAB
UNIVERSIDADE DE SÃO PAULO - RIBEIRÃO PRETO
Conflito de interesse: Não há conflito de interesse

The aim of the present study was to evaluate the effect of the probiotic Bifidobacterium animalis subsp. lactis (B. lacti) HN019 in vivo, as an irrigation solution for root canals in rats' teeth with induced periapical lesion. The animals were divided in four groups, as follows: group I: healthy teeth; group II: periapical lesion; group III: 2.5% sodium hypochlorite; group IV: probiotic, in 7, 21, 42 days. The probiotic irrigating solution was formulated by adding the probiotic at 2.7x 10⁹ CFU in water medium and 2.0% of carboxymethylcellulose. The root canals were irrigated on day 0 and days 3, 7, 14, 21, 28 and 35 days, after periapical lesion induction. The mandible samples were stained in HE and analyzed under optical microscopy to describe the characteristics of the pulp tissue and the apical and periapical regions, in parallel with the morphometric analysis of the extension of the lesion area, osteoclast marking (TRAP), immunohistochemistry to identify markers of the osteoclastogenesis (RANK, RANK-L and OPG) and microbiological analysis using the Brown & Brenn technique. The data were analyzed using software R, version 4.0.0, applying SAS 9.4 and Graph Pad Prism 5, with a significance level of 5%. The histopathological, immunohistochemical and microbiological analysis parameters in probiotic groups were significantly better when compared to groups without periapical lesion and 2.5% sodium hypochlorite (p<0.05).
Therefore, probiotic demonstrated a therapeutic potential on the progression of periapical injuries.
(Apoio: FAPESP  N° 2018/22038-3)

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